LED optical microscope industry changes more obvious advantages than traditional halogen

The LED light source can be customized, provide the correct quality professional task light.

At present in the microscope, the light source is a quartz halogen incandescent lamp, LED is currently in the microscope, because the halogen source usually dissipation 50W-100W. But you can see that the halogen source still have great advantages, they are essentially a blackbody radiator.

This means that they produce a continuous spectrum, no raised place, so you can see any object any visible color, and can be separated from any visible color through the optical filter.

The British manufacturer of LED Plessey component manager Clive Beech said, "halogen benefit is that it is a good source of broad spectrum. The spectrum is very uniform, the color is very good."

Each one has his good points

The optical microscope is not completely. Do the same work some microscope every day, such as white blood cell count, on a narrow range of lighting requirements. The other is general, in these, the required number of more lighting options "". The observer may look directly connected to the microscope eyepiece or camera display.

Researchers sometimes needs a good and accurate color performance, between the requirements they were looking for something with what they are not interested in obtaining high contrast, so it requires the best combination of spectrum and lighting direction.

The first problem is to protect the sample does not influence the halogen heating. Beech said: "it is with a high load of infrared radiation, which is harmful to any tissue samples or organic materials, so you must be filtered out."

LED avoid this layer filter, because the blue core with phosphor technology standard does not produce IR. Plessey optical designer Samir Mezouari said, "the majority of [LED company can simulate the blackbody emission spectrum. But the challenge is how to get the best performance."

Adequate light power has been put in place. Beech said: "our standard white LED 12V 8W 1000 LM camera with 7x7mm package.

The device of the four connection points on the surface of the square tile in a single chip, almost no gap between the transmitter, thereby eliminating the artifacts.

LED change

Despite the need for more current, but the large size of LED chip is also possible, increasing the chip bonding wire size or number of needs. These are the potential problems of lighting of the microscope, even the Kohler lighting is minimized, but the residual image source structure may also be superimposed on the object view.

Even using the bonding wire necessary, LED light source than halogen lamp. For very sensitive samples, LED can be micro flash photography, further reducing heat and infrared.

Beech said: "you cannot remove the halogen in order to reduce the energy input, but you can use the LED. You can shoot the light through always open the camera, with the halogen bulb can not shoot samples can be imaged by LED."

Using LED space lighting more easily. For example, Leica makes the LED ring through the lens reflection microscope, can only produce light from a point of view, you can display surface features.

Because the LED long service life, so LED can permanently built-in optical instruments, to avoid the re arrangement and calibration for replacement of halogen bulb when the steps can also save space, cooling space does not need to replace the bulb fingers and less space.

Contrast enhancement

The first step is to replace halogen with continuous spectrum LED. For contrast enhancement, some microscope contains switchable optical filters, can be replaced with different wavelength LED.

Beech said, "you can't always want to use uniform spectral imaging. For example, with blue light, red light sample group became darker, so it can increase the contrast".

He added, "LED, you can create a wide spectrum of phosphor. Especially in pathology, I will choose the wavelength."

Mezouari said: "imagine the red green blue and white LED, some emission peak has a specific wavelength spectrum. Then, for example, you can turn off the red spectrum, you want to find a specific formula of the sample."

Digital image processing is an important part of the microscope, Mezouari believes that the images taken at different wavelengths of the combined image can be highly enhanced. What kind of wavelength selection of LED depends on the work at hand, but if you use the camera, Beech RGB filter and suggestions will be red and green blue and camera chip matching.

In extreme cases, only use existing LED or halogen to filter out most of the energy from the short wavelength (blue) light, can provide higher resolution. There is some evidence that long-term exposure to intense blue light may cause adverse effects on the human eye.

LED light source

In science more clear before, the operator may be exposed in the light of their own circumstances, then the preventive measures may be limited in the light source halogen analog or green wavelength or longer wavelength. For severe short-term exposure, it has bright biological safety standards.

Full spectrum lighting may cancel is a mistake, because a sample may include only a light source is not consistent with monochromatic materials.

For multi wavelength LED light source, light must somehow be combined. Mezouari said: "you can use a small micro diffuser LED array to obtain hundreds rather than thousands of lumens, but enough."

"If you have enough space, with a single LED and a few centimeters long mixing rod. Or if you have only red green and blue, you can use the prism reflector to